Dual roles of Munc18-1 rely on distinct binding modes of the central cavity with Stx1A and SNARE complex

摘要

The Munc18 central cavity plays a major role in trafficking syntaxin 1 (Stx 1) to the plasma membrane and in activating SNARE-mediated membrane fusion. This paper provides critical insight into the mechanisms of how the Stx1A H3 domain can compete with the SNARE complex for binding the Munc18 central cavity, first inhibiting, and later assisting, SNARE-complex assembly.